Engineering
Understanding™

Founded startup Genomatix LLC during graduate studies at University of Cincinnati (1998)

Performed on-site transgene construction services for Greater Cincinnati Area research labs (1999)

Established independent commercial laboratory in Hamilton County Business Center with ~$300K raised funds (2000)

Executed ~$1M Series A raise for transgene manufacturing expansion and corporate relocation from Cincinnati to the Virginia Tech Corporate Research Center (2004)

Launched cell signaling research tools platform (SubExplorer) to measure phosphorylation dynamics in subcellular compartments (2004)

Formed strategic partnership with RheoGene for RheoSwitch® molecular tools to control gene expression and synergize with the SubExplorer platform (2005)

Raised a $500K Series B-1 Round, changed name from Genomatix to Intrexon Corporation (NewVa Capital Partners) (2005)

Expanded sales and operations with follow-on $1M Series B-2 Round raise (NewVa Capital Partners) (2006)

Raised $5M Series C Round (Third Security, LLC).  Transitioned Intrexon from a research tools and transgene services provider to developer of human therapeutics focused on subcellular modulation of phosphorylation dynamics. (2006)

Acquired RheoGene.  Integrated core technologies to deliver gene therapies focused on immuno-oncology. (2007)

Raised a $25M Series C-2 Round (Third Security, LLC) to focus on accelerating translation of the RheoSwitch® system into human clinical trials and expand library of therapeutic modulators (2007)

Expanded research and development divisions to ~90 scientists and engineers at VA and PA sites to support UltraVector manufacturing division. Managed integration of multidisciplinary teams including DNA/RNA/protein design teams, as well as chemistry, cell biology, viral production, and software development teams. (2007-09)

Co-led preparation, filing, and clinical management of the Phase 1a Study testing safety of the RheoSwitch® Activator Ligand RG-115932 (2008)

Co-led preparation and filing of the Phase 1b, Open Label Trial to Evaluate the Safety, Tolerance, Transgene Function, and Immunological Effects of an Intratumoral Injection of Adenoviral Transduced Autologous Dendritic Cells Engineered to Express hIL-12 in Subjects With Stage III or IV Melanoma (2008)

Led Molecular Engineering Research Unit (2009-13) and Corporate Technology Analysis Unit (2009-19)

Secured $100M Series E Round. Managed technical strategy and diligence components. (2011)

Advanced drug development strategy for Modular Inducible Cancer Immunotherapy (MICI) enabling Exclusive Channel Collaboration with Ziopharm Oncology (2011)

Co-led acquisition of Agarigen core technologies and formation of AgBio operating division and strategic business sector (2011)

Acquired and integrated Cyntellect and GT Life Sciences to form Cell Engineering Unit (2011); managed operations (2011-13)

Advanced genetically-modified (GM) autologous fibroblast drug development strategy enabling Exclusive Channel Collaboration with Fibrocell Science, Inc., including foundational designs for FCX-007, an autologous GM-Cell therapy for treating Recessive Dystrophic Epidermolysis Bullosa (RDEB) (2012)

Co-led design of chimeric antigen receptor T cell (UltraCAR-T®) therapy product development strategy, including invention, acquisition, and optimization of systems required for scalable decentralization of autologous GM-T Cell production (2012-19)

Led scientific communications critical for completion of Intrexon’s initial public offering ($168.3 million) to drive development of programs addressing bioengineering technology opportunities in health, food, energy, environment, and consumer sectors (2013)

Co-led design of therapeutic candidates for treating Friedrich’s Ataxia, enabling formation and Exclusive Channel Collaboration with Agilis Biotherapeutics (2013)

Led Consumer Sector, including launch of US-based ViaGen Pet Services (2014-15)

Co-founded Xogenex (now Precigen Triple-Gene LLC), a gene therapy company focused on developing advanced therapies for complex cardiovascular diseases. (2014)   Managed clinical and business operations. (2018-20)

Acquired GenVec, Inc., a company focused on developing adenoviral-based gene therapies and vaccines. (2017)  Co-managed corporate and platform integration. (2018)

Acquired Genomatix, GMBh to further enhance DNA design and bioinformatics capabilities. (2018). Co-managed corporate and platform integration. (2018-19)

Initiated and Co-led early development of the UltraPorator® gene delivery system approved by FDA for use in autologous chimeric antigen receptor T cell (UltraCAR-T®) clinical trials (2016-2019).

Signal for Targeting Molecules to the Sarco(endo)plasmic Reticulum. Filed in US in 2003, granted as US7071295.

Engineered Cells Expressing Multiple Immunomodulators and Uses Thereof.  Parent application filed in US in 2011; granted as US9492482 and US10046049.

PKD Ligands and Polynucleotides Encoding PKD. Granted in US as US8153598 and US8993531.

Methods of Making Modular Fusion Protein Expression Products.  Granted in US as US8603807.

AKT Ligands and Polynucleotides Encoding AKT Ligands.  Parent application filed in US in 2007; granted as US7943732.

PKA Ligands and Polynucleotides Encoding PKA Ligands.  Granted in US as US8993263.

PKC Ligands and Polynucleotides Encoding PKC Ligands.  Granted in US as US8999666.

Glycogen Synthase Kinase Heteropolyligand Polypeptide. Granted in US as US8729225.

Extracellular-Signal-Regulated-Kinase (ERK) Heteropolyligand Polypeptide.  Granted in US as US8283445.

mTOR Ligands and Polynucleotides Encoding mTOR Ligands.  Parent application filed in US in 2007; granted as US7705122.

MEK Ligands and Polynucleotides Encoding MEK Ligands. Granted in US as US8575304 and as US9006390.

PP1 Ligands. Granted in US as US8455628.

Therapeutic Inhibitors of PAI-1 Function and Methods of Their Use. Parent application filed in 2010 and granted in US as US8431363.

Endoplasmic Reticulum Localization Signals. Parent application filed in US in 2007; granted as US7897394.

Tubulo-Vesicular Structure Localization Signals. Granted in US as US8993742.

Synthetic 5’UTRs, Expression Vectors and Methods for Increasing Transgene Expression. Parent application filed in US in 2010; granted as US8835621.

Methods and Compositions for Diagnosing Disease. Granted in US as US9222121 and US9605316.

Therapeutic Gene-Switch Constructs and Bioreactors for the Expression of Biotherapeutic Molecules and Uses Thereof. Granted in the US as US9724430 and US10314926.

DNA Cloning Vector Plasmids and Methods for Their Use. Parent application filed in US in 2003; granted as US7785871.

Methods for Dynamic Vector Assembly of DNA Cloning Vector Plasmids. Granted in US as US9115361 and US10036026.

Exosome Delivery of Skin Care Peptides. WO2020018926 filed in 2019 with priority to 2018.

Modulating Expression of Polypeptides via New Gene Switch Expression Systems. WO2018132494 filed in 2018 with priority date 2017. Pending in US.

Triple-Gene (formerly Xogenex) presents interim clinical data for INXN-4001 Triple Effector Cardiac Gene Therapy. Thomas Reed. 12th Annual Biotech Showcase 2020 organized by EDB Group, San Francisco, CA. (January 15, 2020)

Xogenex INXN-4001 Triple Effector Cardiac Gene Therapy. Amit Patel and Thomas Reed. 11th Annual Biotech Showcase 2019 organized by EDB Group, San Francisco, CA. (January 9, 2019)

Invited panelist for Council on Competitiveness Briefing to accompany release of “Report on Future of Bioscience and Manufacturing”, Washington DC. (July 25, 2017)

Invited presenter and panelist for National Academies of Sciences, Engineering, and Medicine Committee on Future Biotechnology Products and Opportunities to Enhance Capabilities of the Biotechnology Regulatory System, Washington DC. (April 21, 2016)

Invited panelist for the National Academies of Sciences, Engineering, and Medicine Workshop on “The Disruption Myth and Gaps in the Innovation Ecosystem”, Washington DC. (October 20, 2015)

Invited speaker for ZIOPHARM Oncology Analyst & Institutional Investor Meeting. NASDAQ Auditorium, New York City, New York. (June 23, 2011)

Generation of Mice with a Conditional Allele for the Transforming Growth Factor Beta3 Gene. Thomas Doetschman, Teodora Georgieva, Hongqi Li, Thomas D. Reed, Christina Grisham, Jacqueline Friel, Mark A. Estabrook, Connie Gard, L.P. Sanford, and Mohamad Azhar. Genesis. 2012 January; 50(1):59-66.

ERK Regulates Strain-Induced Migration and Proliferation from Different Subcellular Locations. Gayer CP, Craig DH, Flanagan TL, Reed TD, Cress DE, Basson MD. Journal of Cellular Biochemistry. (2010) March; 109(4):711-25.

Outsourcing Molecular Biology Tasks: A Valuable Business Tool for Accelerating Bioprocessing R&D. Thomas D. Reed. BioContract Services (2004) 1:S25-S30.

Targeted Inhibition of Ca2+/Calmodulin Dependent Protein Kinase II in Cardiac Longitudinal Sarcoplasmic Reticulum Results in Decreased Phospholamban Phosphorylation at Threonine-17. Ji Yong, Bailing Li, Thomas Reed, John Lorenz and John Dedman. Journal of Biological Chemistry. (2003) 278:25063-71.

Replacement of the Muscle-Specific Sarcoplasmic Reticulum Ca(2+)-ATPase Isoform SERCA2a by the Nonmuscle SERCA2b Homologue Causes Mild Concentric Hypertrophy and Impairs Contraction-Relaxation of the Heart. Ver Heyen M, Heymans S, Antoons G, Reed T, Periasamy M, Awede B, Lebacq J, Vangheluwe P, Dewechin M, Collen deS, Sipido K, Carmeliet P, Wuytack F. Circulation Research (2001) 89:838-46.

Changes in Ionic Currents and Beta-Adrenergic Receptor Signaling in Hypertrophied Myocytes Overexpressing G alpha(q). Mitari S, Reed TD and Yatani A. American Journal of Physiology. (2000) 279:H139-48.

The Expression of SR Calcium ATPase and the Na+/Ca2+ Exchanger are Antithetically Regulated during Mouse Cardiac Development and in Hypo/Hyperthyroidism. Reed TD, Babu GJ, Ji Y, Zilberman A, Ver Heyen M, Wuytack F, Periasamy M. Journal of Molecular and Cellular Cardiology. (2000) 32:453-64.

Structure and Organization of the Mouse ATP2A2 Gene Encoding the Sarco(endo)-plasmic Reticulum Ca2+-ATPase 2 (SERCA2) Isoforms. Ver Heyen M and Reed TD (co-first authors), Blough RI, Baker DL, Zilberman A, Loukianov E, Van Baelen K, Raeymaekers, L, Periasamy M and Wuytack F. Mammalian Genome (2000) 11:159-63.

Impaired Cardiac Performance in Heterozygous Mice with a Null Mutation in Sarco(endo)plasmic Reticulum Ca2+-ATPase Isoform 2 (SERCA2) Gene. Periasamy M, Reed TD, Liu LH, Ji Y, Loukianov E, Paul RI, Nieman ML, Miller ML, Riddle T, Duffy JJ, Doetschman, T, Lorenz JN and Shull GE. Journal of Biological Chemistry (1999) 274:2556-62.

Targeted Overexpression of the Sarcoplasmic Reticulum Ca2+-ATPase Increases Cardiac Contractility in Transgenic Mouse Hearts. Baker DL, Hashimoto K, Grupp IL, Ji Y, Reed T, Loukianov E, Grupp G, Bhagwat A, Hoit B, Walsh R, Marban E and Periasamy M. Circulation Research (1998) 83:1205-14.

Sarco(endo)plasmic Reticulum Ca2+ ATPase Isoforms and Their Role in Muscle Physiology and Pathology. Loukianov E, Ji Y, Baker DL, Reed T, Babu J, Loukianov T, Greene A, Shull G and Periasamy M. Annals of the New York Academy of Sciences (1998) 853:251-59.

A Novel E Box/AT-Rich Element is Required for Muscle-Specific Expression of the Sarcoplasmic Reticulum Ca2+-ATPase (SERCA2) Gene. Baker DL, Dave V, Reed T, Misra S and Periasamy M. Nucleic Acids Research (1998) 26:1092-98.

Multiple Sp1 Binding Sites in the Cardiac/Slow Twitch Muscle Sarcoplasmic Reticulum Ca2+-ATPase Gene Promoter are Required for Expression in Sol8 Muscle Cells. Baker D, Dave V, Reed T and Periasamy M. Journal of Biological Chemistry (1996) 271:5921-28.

Characterization of the Mouse Cardiac/Slow-Twitch Muscle-Enhanced Sarco(endo)plasmic Reticulum Calcium ATPase (SERCA2) Gene. Thomas D. Reed. Doctor of Philosophy Dissertation (1998) University of Cincinnati College of Medicine; Cincinnati, Ohio.

Safety of First-in-Human Triple Gene Therapy for Heart Failure Patients. Ewa Jaruga-Killeen, David Bull, Kapildeo Lotun, Timothy Henry, Gregory Egnaczyk, Thomas Reed, Amit Patel. American Heart Association, Scientific Sessions 2019.

Human Cardiac iPSC Evaluation of Novel Triple Effector Gene Therapy for Dilated Cardiomyopathy. Dimki Patel, Joseph Wu, Thomas Reed, Amit Patel. American Society of Gene and Cell Therapy, Annual Meeting 2019.

Minimally Invasive Novel Triple Effector Gene Therapy for Adriamycin Cardiomyopathy. Paul Grayburn, Dimki Patel, Shuyuan Chen, Thomas Reed, Amit Patel. American Society of Gene and Cell Therapy, Annual Meeting 2019. 

Retrograde Delivery of Novel Triple Effector pDNA Gene Therapy for Heart Failure. Thomas Reed, Dimki Patel, Eric Rodenberg, Brad Shirley, Mark Johnson, Amit Patel. American Society of Gene and Cell Therapy, Annual Meeting 2019.

First Human Experience with Novel Triple Effector Gene Therapy for Heart Failure – INXN-4001. Thomas Reed, Amy Lankford, David Bull, Ewa Jaruga-Killeen, Amit Patel. American Society of Gene and Cell Therapy, Annual Meeting 2019.

Ad-RTS-hIL-12 + Veledimex Regulation of IL-12 Expression in Advanced Breast Cancer and Melanoma. John Nemunaitis, Gerald Linette, Haythem Ali, Troy Guthrie, Francois Lebel, John Barrett, Thomas Reed, Suma Krishnan, Jonathan Lewis, Larry Norton. AACR Tumor Immunology 2014.

Regulated Intratumoral Expression of IL-12 in combination with cytotoxic agents as a strategy for the treatment of metastatic breast cancer. John Nemunaitis, Heather McArthur, Clifford Hudis, Thomas Reed, Samuel Broder, Francois Lebel, John Barrett, Jonathan Lewis, Larry Norton. San Antonio Breast Cancer Symposium 2013.

A Phase I Open-Label Study of Ad-RTS-hIL-12, an Adenoviral Vector Engineered to Express hIL-12, in Combination with an Oral Activator Ligand in Subjects with Unresectable Stage III/IV Melanoma. G.P. Linette, O. Hamid, E.D. Whitman, J.J. Nemunaitis, J. Chesney, S.S. Agarwala, A. Starodub, J.A. Barrett, A. Marsh, L. Martell, A. Cho, T.D. Reed, H. Youssoufian, A. Vergara Silva. American Society of Clinical Oncology Annual Meeting 2013.

Pharmacodynamics and Functionality of RheoSwitch® Regulated Immunostimulatory and Immunomodulatory Proteins, Expressed from a Multigenic Embedded Cellular Bioreactor following Intramuscular Electroporation in Mice. Pooja Agarwal, Stephen Schauer, Xiaohong Ma, Jacques Plummer, Tim Chan, Lindsay Williams, M. Kaloss, John Barrett, R. Einstein, Laurent Humeau and Thomas Reed. AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics 2013.

Integration of a Modularized Protein Engineering Technology and the RheoSwitch Therapeutic System® (RTS®) Platform to Develop High Affinity Trastuzumab Single Chain Variable Fragment-Fc Proteins for Gene Therapy Applications. C. Reed, V. Dailey, A. Elayadi, L. Williams, D. Schneider, T. Reed, J. Lewis, R. Einstein and S. Broder. AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics 2013.

Regulated Immunodulators Expression Using the RheoSwitch Therapeutic System® Platform in Human Mesenchymal Stem Cells. T. Chan, A. Prabhu, A. Elayadi, L. Williams, V. Dailey, K. Elliot, T.S Snipas, J. Carson, J. Lewis, S. Schauer, R. Einstein, J. Rieger, L Humeau and T. Reed. AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics 2013.

U.S. Council on Competitiveness Technology Leadership & Strategy Initiative Committee (2016-19).  The TLSI is a cross-sectoral, results-driven “action tank” making the business case for strategic, prioritized investments in the research, talent and infrastructure necessary for tech-based innovation; chaired by Boeing Chief Technology Officer Greg Hyslop, Lockheed Martin CTO Keoki Jackson and Deere & Company VP for Advanced Technology & Engineering Klaus Hoehn and comprising a peer group of CTOs, CSOs and heads of research at leading companies, universities and national laboratories. Click here to learn more about TLSI mission.

Wright State University Contributor

• Co-sponsored development of the College of Liberal Arts’ (COLA) “Change the World”

• Co-sponsored entrepreneurial programs led by the College of Science and Mathematics (COSM)

• Co-sponsored collaborative initiative between COSM and COLA’s Departments of Theatre, Dance, and Motion Pictures focused on empowering incoming freshmen

Cincinnati Life Science CEO Roundtable Member (2002-04)

Cincinnati Chamber of Commerce, Regional Entrepreneurial Network, “Voice of the Consumer” High-tech Leadership Committee Member (2002)

Policy Steering Committee Advisor, Chris Monzel for Cincinnati City Council Campaign (2000)

Christ’s Community in College Hill, Trustee (1995-2000), Chairperson (1996-97)

• Assisted in operational overhaul and re-budgeting process to enable inter-denominational support for spiritual, physical, emotional, financial, and social needs of residents living in College Hill, Ohio

• Coordinated community outreach programs for under-privileged youth and administered volunteer programs to strengthen community, enhance interfaith relationships, and address local challenges including food disparities